Dried blood spot versus venous blood sampling for phenylalanine and tyrosine

Estimation of blood phenylalanine from a dried blood spot using the Guthrie test.

Lactate measurement: arterial versus venous blood sampling

Lactate is the end product of the metabolic process of glucose utilization, known as anaerobic glycolysis, which occurs in the cytoplasm of all cells. In well-oxygenated tissues this lactate is metabolized further, but if tissues are inadequately oxygenated, lactate accumulates locally and blood concentration increases. As a blood marker of tissue hypoxia, lactate measurement has long-establish...

Dried blood spot sampling: practical considerations and recommendation for use with preclinical studies.

At present it is necessary to use animals to generate toxicokinetic data as part of preclinical safety studies. However, ethical standards require animal use to be carefully monitored and the principles of the 3Rs: replacement, reduction and refinement, to be considered and applied. Use of dried blood spot (DBS) samples, typically 10 to 20 µl, rather than the larger blood volumes required to ob...

Dried Blood Spot Sampling for Hepatitis B Virus Serology and Molecular Testing

BACKGROUND AIMS Dried blood spots (DBS) on filter paper have been successfully used to diagnose and monitor several infectious diseases. The aim was to investigate the performance of DBS in hepatitis B virus (HBV) diagnosis using commercial tests in comparison to standard methods. METHODS Paired DBS and plasma samples were collected from 200 patients: 100 patients with HBsAg negative status a...

Comparability of HbA1c and lipids measured with dried blood spot versus venous samples: a systematic review and meta-analysis

BACKGROUND Levels of haemoglobin A1c (HbA1c) and blood lipids are important determinants of risk in patients with diabetes. Standard analysis methods based upon venous blood samples can be logistically challenging in resource-poor settings where much of the diabetes epidemic is occurring. Dried blood spots (DBS) provide a simple alternative method for sample collection but the comparability of ...